Biomedicum Protein Production and Purification Core Facility (B3P) B3P
Production and
Purification Core Facility
Biomedicum Protein Production and Purification (B3P) Logo
Michael Jeltsch
PhD, Adjunct Professor
Academy Research Fellow
Research Programs Unit
Room B318a
P.O. Box 63 (Haartmaninkatu 8)
00014 University of Helsinki
phone +358 2 941 25514
fax +358 2 941 25510
Olli Ritvos
MD/PhD, Adjunct Professor
Instruct Protein production services facilitator
Medicum, room B132b
P.O. Box 63 (Haartmaninkatu 8)
FIN-00014 University of Helsinki
phone +358 2 941 25284
Arja Pasternack
PhD, Senior Researcher
Protein production specialist
Medicum, room B132b
P.O. Box 63 (Haartmaninkatu 8)
FIN-00014 University of Helsinki
phone +358 2 941 25284


Alma-intranet Flammaintra

Large equipment

Äkta Avant

Äkta Explorer (will be discontinued)

WAVE Bioreactor 20/50EHT

Malvern PEAQ-iTC

Savant SpeedVac SPD121P


Columns and resins

Prepacked columns

We have a modest choice of prepacked columns. Since they are not cleaned after every use, their condition (especially that of the rarely used columns) is sometimes unknown. They might require cleaning/sanitation before use.

Ion exchange
  • 1x Mono Q HR 5/5
  • 1x Mono Q 5/50 GL
  • 1x Resource Q 1ml
  • 1x Mono S HR 5/5
  • 1x Mono S 5/50 GL
  • 1x Mini S 4.6/50 PE
  • 1x Resource S 1ml
Reverse phase
  • PepRPC HR 5/5

Single-use columns

A good alternative for many applications are single use columns, which you need to provide yourself. We have a small stock of some of the more frequently used types which you can borrow if you are in a hurry.

Ion exchange
  • 1ml and 5ml HiTrap Q HP
  • 1ml HiTrap S HP
  • 1ml and 5ml HiTrap Q HP
  • 1ml HiTrap S HP
Metal chelate
  • 1ml HisTrap HP

Empty columns

The empty columns are vailable for packing, but occassionally one or the other might be packed with expesive media for multiple use by some user and is therefore occupied for longer periods of time.

Tricorn 5mm
  • 2x 5/50 columns with standard adaptor units
  • 1x 5/150 glass tube
  • 1x packing connector (to make one 100mm column out of two 50mm colums or for column filling)
Tricorn 10mm
  • 1x 10/50 glass tube
  • 2x adapter unit
HR 5mm
  • 2x 5/50 columns
HR 10mm
  • 1x 10/10 column
XK 16mm
  • 2x XK16/20 columns including column packing kit


Some of the bulk media is available for free as the smallest available package sizes are so large that the media anyway expires before anybody can use it to the end.

Ion exchange
  • SP Sepharose Fast Flow
  • DEAE Sepharose CL-6B
Gel filtration/size exclusion
  • Sephadex G-100, G-50, G-15
  • Sephacryl S-500 HighRes
  • Sepharose 4 FastFlow
Metal chelate
  • Ni2+ NTA Superflow
  • Ni2+ NTA Agarose
  • Chelating Sepharose Fast Flow
  • Heparin Sepharose 6 Fast Flow
  • IgG Sepharose 6 Fast Flow
  • ConA Sepharose
  • CNBr-activated Sepharose 4B
  • Ceramic Hydroxyapatite

Enzyme lists

We still use fairly often old-fashioned restriction enzyme cloning to assemble our expression constructs. If you only need a few microlitres (or if you are waiting for your enzyme to arrive and want to borrow something for the initial fragment generation), please ask!
* Displaying our enzyme list works only inside the Helsinki University network since it polls our intranet server directly!

Restriction enzymes*

Other enzymes*